Program Introduction
Chapter 1: Tools of the trade
Chapter 2A: How do you begin to clone a gene?
Chapter 4A: Making sure you’ve got a recombinant plasmid
Chapter 5A: Getting recombinant plasmids in bacteria

AMGEN Biotechnology Lab
Assignment Sheet Due Date:

Date Assigned
Date
Due
Chapter
Section Heading
Question Type
Page
Question #
Score
3/3

3/6
Intro
What is genetic engineering?
CONSIDER
A5, A6, A11
1 – 4

3/6
3/7

2A
Introduction
WHAT DO YOU ALREADY KNOW
C3
1 – 4

3/6
3/7
2A
Plasmids and Restriction Enzymes
CONSIDER
C5 – C7
1 – 4

3/8
in class
2A
Clone That Gene
STOP AND THINK + plasmid
C9
1

3/8
3/13
2A
Clone That Gene
ACTIVITY QUESTIONS
C11
1 – 3

3/7
3/8
1
Introduction
WHAT DO YOU ALREADY KNOW
A17
1 – 2

3/7
3/8
1
Lab 1.1: How to use a micropipette
BEFORE THE LAB
A19
1 – 2

3/7
3/8
1
Lab 1.1: How to use a micropipette
STOP AND THINK + drawings
A21
1 – 2

3/7
3/8
1
The Genetic Engineering Process
CONSIDER
A22
1

3/8
3/13
1
Lab 1.2: Gel Electrophoresis
BEFORE THE LAB
A25
1 – 2

3/13
3/14
1
Lab 1.2: Gel Electrophoresis
STOP AND THINK + gel results
A29
1 – 3

3/14
3/15
1
Chapter 1 Questions
CHAPTER 1 QUESTIONS
A30
1 – 4

3/13
3/14
2A
Lab 2A: Preparing to verify the rfp gene: Digesting the pARA-R plasmid
STOP AND THINK
C13
1

3/13
3/14
2A
Lab 2A: Preparing to verify the rfp gene: Digesting the pARA-R plasmid
BEFORE THE LAB
C13
1 – 4

3/15
3/16
2A
Lab 2A: Preparing to verify the rfp gene: Digesting the pARA-R plasmid
STOP AND THINK
C15 – C16
1 – 2

3/15
3/16
2A
Chapter 2A Questions
CHAPTER 2A QUESTIONS
C17
1 – 5

3/15
3/16
4A
Introduction
WHAT DO YOU ALREADY KNOW
C23
1 – 2

3/15
3/16
4A
Verification
CONSIDER
C26, C28
1 – 2

3/16
3/17
4A
Lab 4A: Verification of the recombinant plasmid using gel electrophoresis
BEFORE THE LAB
C29
1 – 3

3/16
3/17
4A
Lab 4A: Verification of the recombinant plasmid using gel electrophoresis
STOP AND THINK + gel picture
C30, C32
1 – 3

3/20

4/3

4A
Chapter 4A Questions
CHAPTER 4A QUESTIONS
(not from manual)
C33
1 – 8

3/20
3/21
5A
Introduction
WHAT DO YOU ALREADY KNOW
C37
1 – 4

3/20
3/21
5A
Transforming bacteria with recombinant plasmids
CONSIDER
C38 – C39
1 – 2

3/20
3/21
5A
Lab 5A: Transforming bacteria with the pARA-R plasmid
BEFORE THE LAB
C43 – C44
1 – 4

3/23

4/3

5A
Lab 5A: Transforming bacteria with the pARA-R plasmid
STOP AND THINK
C47
1 – 4

3/23

4/3

5A
Lab 5A: Transforming bacteria with the pARA-R plasmid
Results + Transformation Efficiency
----
1 – 4

3/23

4/3

5A
Lab 5A: Transforming bacteria with the pARA-R plasmid
CHAPTER 5A QUESTIONS
C50
1 – 6









Total Lab Score:



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